Ross Street Presbyterian Church was founded in 1864 by members of the South Third Street Presbyterian Church. Early supporters included Sylvester M. Beard, Eli Beard and Halsey Fitch.
Cornerstone laid in September 1889. The current structure replaced an older structure dating to about 1874 that was located to the east on Hooper Street. The old church was built with the intention of later being converted to a school, and therefore had beam pockets built into the walls for the installation of two new floors at a future date. (That church/school was later replaced with a new school.)